Journal: Chemical reviews

Article Title: ImmunoPET: Concept, Design, and Applications

doi: 10.1021/acs.chemrev.9b00738

Figure Lengend Snippet: ImmunoPET imaging of ovarian cancers with a bispecific radiotracer 89Zr-DFO-REGN4018. (a) 89Zr-DFO-REGN4018 immunoPET/CT imaging of humanized tumor-bearing mice showed the distribution of the tracer to the spleen (yellow arrow), lymph nodes (green arrow), and tumor (red arrow). (b) Blocking with a MUC16 parental antibody reduced the tumor uptake of 89Zr-DFO-REGN4018 without influencing the spleen and lymph node uptake. (c) Blocking with an anti-CD3 antibody substantially reduced the spleen and lymph node uptake of 89Zr-DFO-REGN4018 without influencing the tumor uptake. Reproduced with permission from ref 604. Copyright 2019 American Association for the Advancement of Science.

Article Snippet: ImmunoPET imaging with a 89 Zr-labeled antimouse CD3 antibody (clone 17A2; R&D Systems) revealed a correlation between high tumor uptake of the radiotracer and better therapeutic response of anticytotoxic T lymphocyte antigen 4 (CTLA-4) therapy in a preclinical colorectal cancer model. 788 However, significant liver accumulation of the radiotracer was found on immunoPET images, which was explained as liver clearance of the radiolabeled antibody.

Techniques: Imaging, Blocking Assay

Journal: Chemical reviews

Article Title: ImmunoPET: Concept, Design, and Applications

doi: 10.1021/acs.chemrev.9b00738

Figure Lengend Snippet: Representative Clinical-Stage ImmunoPET Imaging Probes a

Article Snippet: ImmunoPET imaging with a 89 Zr-labeled antimouse CD3 antibody (clone 17A2; R&D Systems) revealed a correlation between high tumor uptake of the radiotracer and better therapeutic response of anticytotoxic T lymphocyte antigen 4 (CTLA-4) therapy in a preclinical colorectal cancer model. 788 However, significant liver accumulation of the radiotracer was found on immunoPET images, which was explained as liver clearance of the radiolabeled antibody.

Techniques: Imaging

Journal: The Journal of clinical endocrinology and metabolism

Article Title: Association of postpartum thyroid dysfunction with antepartum hormonal and immunological changes.

doi: 10.1210/jc.2002-021219

Figure Lengend Snippet: FIG. 1. Graphical output from the FACScan analyzer (Becton Dickinson and Co.). Before analysis lymphocytes were stained with R-phyco- erythrin-Cy5 mouse antihuman CD3 fluorescein isothiocyanate-conjugated mouse antihuman CD4 together with either phycoerythrin- conjugated rat antihuman IL-4 or phycoerythrin-conjugated mouse antihuman IFN-. The output was gated on the basis of side scatter and CD3 staining. Shown are control cells in the left panels and stimulated cells in the right panels. Lymphocytes gated for right-angle light scatter and CD3 staining (a and b), CD4 expression (c and d), IL-4 expression (c–f), and IFN- expression (e and f) are shown.

Article Snippet: The culture plate was placed in a humidified incubator at 37 C, gas phase 5% CO2 in air, for 4 h. Aliquots (350 l) from the stimulated and unstimulated wells were transferred into fluorescenceactivated cell sorter (FACS) tubes and the surface antigen (CD3 and CD4) was stained by adding 5 l conjugated antibody (R-phycoerythrin-Cy5 conjugated mouse antihuman CD3, DAKO Corp. Ltd., Ely, UK; fluorescein isothiocyanate-conjugated mouse antihuman CD4, Serotec Ltd., Oxford, UK) and incubated in the dark for 30 min.

Techniques: Staining, Control, Expressing